Scalable isolation of RNA and microRNA from a variety of samples

The Applied Biosystems™ MagMAX ™ mirVana™ Total RNA Isolation Kit is designed to extract and purify RNA. The isolation of total RNA, as well as small RNAs such as microRNA (miRNA), from a wide variety of sample matrices is possible with this kit. This product uses MagMAX™ magnetic bead technology to help ensure reproducible recovery of high-quality RNA that is suitable for a broad range of applications, including TaqMan™ miRNA Detection Assays.

The magnetic bead–based purification format allows you to easily process 6 to 96 samples at once when used with the Thermo Scientific™ KingFisher™ Duo Prime or Thermo Scientific™ KingFisher™ Flex Magnetic Particle Processors. Alternatively, samples can be processed manually using a magnetic stand.

Features of the MagMAX mirVana Total RNA Isolation Kit include:

  • Automation-ready, phenol-free extraction
  • Streamlined protocols for numerous non-invasive biological samples as well as tissues and cell culture
  • Recovery of pure miRNA compatible with miRNA-Seq and qRT-PCR methods such as the TaqMan™ Advanced miRNA cDNA Synthesis Kit and other TaqMan™ advanced miRNA assays

One kit to isolate true total RNA from diverse sample types

Why purchase multiple purification kits to isolate all the RNAs present in your different sample types? The MagMAX™ mirVana™ Total RNA Isolation Kit eliminates the headache of ordering “specialized” kits because it has been optimized for use with most biological samples. No longer are separate kits needed to isolate microRNA from plasma and solid tissue samples.

The kit is designed for both low- and high-throughput isolation of true total RNA that covers the full range of RNAs present in cells, from small RNAs such as microRNA, to noncoding RNA, messenger RNA, and ribosomal RNA. The phenol-free, magnetic bead format makes the kit fully amenable to automated RNA isolation from the following samples:

 
  • Serum/plasma
  • Whole blood
  • Tissue
  • Cell culture
  • Urine
 

Benefits of MagMAX™ magnetic bead–based purification

The MagMAX™ family of products is designed to provide consistent and reliable nucleic acid purification using silica–magnetic particle technology. Magnetic beads offer many benefits for isolating RNA from cells, tissue, or blood, and viral RNA from cell-free samples. The technology at the core of the MagMAX™ product line:

  • Binds RNA more efficiently than silica-membrane methods
  • Offers higher, more consistent RNA yields
  • Allows RNA to be eluted in as little as 20 µL of nuclease-free water, resulting in more concentrated RNA

Find out more about KingFisher Magnetic Particle Processors

Video: How to isolate total RNA using magnetic bead–based technology

Learn how to achieve rapid, quantitative isolation of total RNA in both manual and automated workflows with the MagMAX™ MirVana™ Total RNA Isolation Kit.

Resources

Nucleic Acid Purification and Analysis Support Center
Find tips, troubleshooting help, and resources for your nucleic acid purification & analysis applications.

For Research Use Only. Not for use in diagnostic procedures.